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An anchialine pool is a landlocked body with a subterranean connection to the ocean. Anchialine pools are a feature of coastal aquifers which are density stratified, with the water near the surface being fresh or brackish, and saline water intruding from the coast below at some depth. Depending on the site, it is sometimes possible to access the deeper saline water directly in the anchialine pool or sometimes it may be accessible by cave diving.

Water levels in anchialine pools often fluctuate with tidal changes due to the coastal location and the connection with the ocean. The range in water levels fluctuations will be decreased (damped) and delayed compared to the range and time observed for the adjacent tide. The primary controls on the damping and lag are the distance from the coast, and the hydraulic conductivity of the geological materials.

Anchialine pools are extremely common worldwide especially along neo-tropical coastlines where the geology and aquifer system are relatively young, and there is not much soil development. Such conditions occur notably where the bedrock is limestone or recently formed volcanic lava. Many anchialine pools are found on the coastlines of the island of Hawaiimarker, and on the Yucatán Peninsulamarker, where they are locally called cenotes.

Ecological studies of anchialine pools frequently identify regionally rare and sometimes endemic species living in them.

In Hawaii, the pools are home to mostly the ōpae ula (Hawaiian Shrimp, Halocaridina rubra).

In karst anchialine pools and the caves these may be connected to, the fauna are diverse and include: crustaceans including remipedia and copepods, and among the vertebrates are several species of blind cave fish and eels.

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